RNeasy 96 Universal Tissue Kit (4)，74881，Qiagen，凯杰

The RNeasy Plus Universal Mini Kit integrates fast, convenient RNA purification with effective elimination of genomic DNA. Optimized protocols enable purification of high-quality RNA from any type of tissue, even difficult-to-lyse tissues. The kit includes QIAzol Lysis Reagent for lysing fatty tissue and other types of tissue and RNeasy spin columns for purifying high-quality RNA. The RNeasy Plus Universal Mini Kit can be automated on the QIAcube Connect. The RNeasy 96 Universal Tissue Kit enables total RNA purification in 96-well format from any tissue. Tissues are efficiently lysed and homogenized in QIAzol Lysis Reagent, and high-quality RNA is purified using silica-membrane RNeasy 96 plates. Manual purification is carried out using the QIAGEN Centrifuge 4-16KS and, optionally, the QIAvac 96 vacuum manifold. Automated purification using the related RNeasy 96 Universal Tissue 8000 Kit is performed on the BioRobot Universal System. Tissue samples can be conveniently stabilized using Allprotect Tissue Reagent, and efficiently disrupted using the TissueLyser II. Nonfatty tissues can be stabilized using the RNAprotect Tissue Reagent.

• High yields of total RNA from all tissues
• Fast non-enzymatic removal of genomic DNA
• Streamlined QIAzol lysis and RNeasy 96 purification
• Pure, high-quality RNA without phenol contamination
• High-performance RNA for all downstream applications

RNA purified using RNeasy Plus Universal Kits is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as RNA-seq, quantitative real-time RT-PCR. The purified RNA can also be used in other applications.

The combination of QIAzol and RNeasy technologies results in highly pure RNA without phenol carryover. RNA purified using the RNeasy 96 Universal Tissue Kit is suitable for all downstream applications, including RNA-seq, real-time RT-PCR and array analysis.