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General description
Tubulin, the dimeric subunit of microtubules, is submitted to post translational modifications including cyclic tyrosine removal and addition at the COOH terminus of the alpha subunit. The cycle involves two enzymes, TTL (Tubuline Tyrosine Ligase) and Tubulin carboxypeptidase, and generates two major forms: tyrosinated tubulin and detyrosinated tubulin (Glu-tubulin). Removal of the COOH-terminal Glu residue from the alpha chain of Glu-tubulin generates the delta2-tubulin form.
Immunogen
Peptide corresponding to the C-terminal of delta 2 tubulin.
Application
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Tubulin Antibody, δ 2 is validated for use in IH(P), WB, IC, IH for the detection of Tubulin.
Western blot: 1:1,000
Immunohistochemistry and EM on either frozen or paraffin embedded, fixed tissue (Mialhe et al. 2001): 1:500
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
The antibody specifically recognizes the delta 2 form of the tubulin alpha chain (Paturle-Lafanechere et al. 1991).
SPECIES REACTIVITY:
Mammals, sea urchin, plants, not yeast (Lafanechere et al. 1994).
Physical form
Affinity purified immunoglobulin. Liquid in PBS.
Preparation Note
Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | sea urchin, echinoderm, plant |
| should not react with | Saccharomyces cerevisiae |
| species reactivity (predicted by homology) | mammals |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| NCBI accession no. | NM_016261.2 |
| UniProt accession no. | Q9UJT1 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TUBD1(51174) |
| 长度(mm) | |
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