Anti-TNF-α Rabbit pAb，1MG，654250-1MGCN，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
Recognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.

Immunogen

recombinant, human TNF-α

Application

ELISA (see comments)

Frozen Sections (see comments)

Immunoblotting (see comments)

Neutralization Studies (see comments)

Paraffin Sections (see comments)

Radioimmunoassay (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Positive Control
L929 cells

Other Notes

1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

技术参数 Specifications

biological source	rabbit
Quality Segment	100
antibody form	purified antibody
antibody product type	primary antibodies
clone	polyclonal
form	liquid
does not contain	preservative
species reactivity	human, mouse (weakly)
manufacturer/tradename	Calbiochem^®
storage condition	OK to freeze,avoid repeated freeze/thaw cycles
dilution	(ELISA Frozen Sections Immunoblotting Neutralization Studies Paraffin Sections Radioimmunoassay )
impurities	≤1 EU/mg Endotoxins
isotype	IgG
shipped in	wet ice
storage temp.	−20°C
target post-translational modification	unmodified
Gene Information	human ... TNF(7124)

长度(mm)
宽度(mm)
高度(mm)
重量(kg)