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General description
Death-inducer obliterator 1 (UniProt Q9BTC0; also known as DATF-1, Death-associated transcription factor 1, DIO-1, hDido1) is encoded by the DIDO1 (also known as C20orf158, DATF1, KIAA0333) gene (Gene ID 11083) in human. The DIDO1 gene encodes four alternatively spliced variants (DIDO1/isoform 2, DIDO2/isoform 1, DIDO3/isoform 4, isoform a/isoform 3) implicated to be involved in apoptosis and development. The longest and most widely expressed DIDO3 is assigned as the canonical isoform. DIDO3 is a nuclear protein that forms part of the spindle assembly checkpoint (SAC) and is necessary for correct chromosome segregation in somatic and germ cells. Dido3-knockout compromises differentiation of murine embryonic stem cells (mESCs) in vitro and of epiblast cells in vivo, resulting in early embryonic death at around day 8.5 of gestation. On the other hand, exogenous expression of Dido1 isoform in mESCs is reported to inhibit LIF withdrawal-induced differentiation, while Dido1 knockdown facilitate mESCs differentiation.
~260/130/79/70/66 observed. 59.46 kDa (isoform 2; DIDO1), 129.2 kDa (isoform 1; DIDO2), 243.9 kDa (isoform 4; DIDO3), 61.35 kDa (isoform 3; isoform a) calculated. Uncharacterized band(s) may appear in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding an N-terminal sequence of human DIDO1.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DIDO immunoreactivity in human kindey, human prostate cancer and human cerebellum tissue.
This Anti-Pan DIDO Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DIDO.
Biochem/physiol Actions
This polyclonal antibody detects an N-terminal epitope present in all four spliced isoforms of human DIDO1 reported by UniProt (Q9BTC0).
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected DIDO isoforms in 10 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | nonhuman primates (based on 100% sequence homology), mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_071388 |
| UniProt accession no. | Q9BTC0 |
| target post-translational modification | unmodified |
| Gene Information | human ... DIDO1(11083) |
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