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General description
Calcium/calmodulin-dependent protein kinase type II subunit beta (UniProt: P08413; also known as EC: 2.7.11.17, CaM kinase II subunit beta, CaMK-II subunit beta) is encoded by the Camk2b gene (Gene ID: 24245) in rat. CaM kinase II is one of the most widely studied multi-functional enzyme. It is found in high concentrations in the brain and in some regions it may constitute up to 2% of the total protein content. CaM kinase II is a complex of about 12 subunits, which exist in four differentially expressed forms (alpha, beta, gamma, and delta). CaMKII beta contains an auto-inhibitory domain (aa 283-293), a calmodulin-binding domain (aa 291-301) and a unique C-terminal association domain responsible for oligomerization. It is activated Ca2+/calmodulin and binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr-287 which turns the kinase in a constitutively active form and confers to the kinase a Ca2+-independent activity that persists even after withdrawal of calcium. Phosphorylation of Thr-287 locks the kinase into an activated state. CaM Kinase II typically recognizes the phosphorylation site sequence Arg-X-X-Ser/Thr. It prefers arginine in the P3 position, which is reported to make an electrostatic contact with Glu96 in the catalytic core.
~60 kDa observed; 60.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
A linear peptide corresponding to 10 amino acids surrounding phopho serine 331 of rat CaM Kinase II beta.
Application
Anti-phospho-CaMKII beta (Ser331), Cat. No. ABS2107, is a highly specific rabbit polyclonal antibody that targets Calcium/calmodulin-dependent protein kinase type II subunit beta (CaMK-II subunit beta) and has been tested for use in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT or mutants (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
Biochem/physiol Actions
This rabbit polyclonal antibody detects beta subunit of CaM Kinase II when phosphorylated on Ser331.
Analysis Note
Evaluated by Western Blotting in HEK293T cells transfected with CaMKII WT or mutant S331A.
Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT, but not the mutant S331A.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat |
| species reactivity (predicted by homology) | human (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μL |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_068507 |
| UniProt accession no. | P08413 |
| target post-translational modification | phosphorylation (pSer331) |
| Gene Information | rat ... Camk2B(24245) |
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¥ 2528.88
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¥ 1596.77
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¥ 2019.57
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¥ 3812.28
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