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General description
Akt（蛋白激酶B）是一种丝氨酸/苏氨酸激酶，是涉及细胞增殖、凋亡、血管生成和糖尿病的信号转导途径的关键酶。据报道，在哺乳动物中，Akt的三种同种型（α，β，γ 或Akt 1、2、3）显示高度同源性，但其调节性磷酸化位点略有不同。Akt α 是大多数组织中的主要同工型，Aktβ在胰岛素反应性组织中表达量**，而Aktγ在脑组织中表达量丰富。每个Akt同种型由三个功能不同的区域组成：一个N末端pleckstrin homology（PH）结构域（它可提供一个脂质结合模块以将Akt引导至PIP2和PIP3上）、一个中央催化结构域和一个C末端疏水序列。
60kda
Application
使用此抗Akt/PKB抗体，PH结构域，克隆SKB1，检测Akt/PKB，经过验证可用于FC、IC、IP&WB。
Physical form
免疫亲和力蛋白G纯化的免疫球蛋白，溶于0.02 M磷酸盐缓冲液（pH 7.6）、0.25M NaCl、0.1％叠氮化钠和30％甘油中。
形式：纯化
Analysis Note
对照
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。 加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以 减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
已通过免疫印迹法对A431细胞的RIPA裂解物进行了常规评估
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | SKB1, monoclonal |
| species reactivity | rat, human, mouse |
| packaging | antibody small pack of 25 μg |
| manufacturer/tradename | Upstate® |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| web metadata keyword.default | Akt antibody for angiogenesis studies,Akt antibody for diabetes research applications,Akt isoform-specific antibody,Akt protein kinase B antibody immunoprecipitation,Akt1 antibody clone SKB1 for research,Akt2 antibody for cell proliferation research,Anti-Akt PKB antibody clone SKB1,Anti-Akt PKB antibody,PH domain Akt antibody flow cytometry,Sigma-Aldrich Anti-Akt antibody for western blot,anti-Akt antibody for apoptosis signaling,anti-Akt antibody for signal transduction studies,custom supply Anti-Akt PKB antibody,high purity Anti-Akt antibody for immunocytochemistry,monoclonal Anti-Akt PKB antibody |
| NCBI accession no. | NM_005163 |
| UniProt accession no. | P31749 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Akt1(11651) |
| 长度(mm) | |
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