Anti-MMP-14 Antibody,100UL,AB8345,Sigma

销售价: ¥ 4837.50 / 件
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订货号 : 0BV4667
品牌型号 : Sigma AB8345
货期 : 询货期
最小订货量 : 1件
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产品介绍 Product Description

Immunogen

Catalytic domain of MT1-MMP


Application

Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.
Immunocytochemistry: 5-10 μg / ml

Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.

Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.

FACS Analysis: 5 μg/ mL (1 million cells per mL)

EIA: 1:10,000

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs


Biochem/physiol Actions

The antibody recognizes native and recombinant proteins.


Physical form

Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.


Preparation Note

Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.


Analysis Note

Control
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.

Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma


Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerabbit
Quality Segment100
conjugateunconjugated
antibody formserum
antibody product typeprimary antibodies
clonepolyclonal
species reactivityhuman
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable
NCBI accession no.NM_004995.2
UniProt accession no.P50281
shipped indry ice
target post-translational modificationunmodified
Gene Informationhuman ... MMP14(4323)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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