PKCδ, Human, Recombinant, S. frugiperda,5UG,539672-5UGCN,Sigma

销售价: ¥ 4636.67 / 件
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订货号 : 0BV3222
品牌型号 : Sigma 539672-5UGCN
货期 : 询货期
最小订货量 : 1件
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产品介绍 Product Description

General description

Full-length, recombinant, human PKCδ expressed in S. frugiperda insect cells using a baculovirus expression system. PKC δ is classified as a novel PKC isozyme because it does not exhibit calcium dependence and it is activated in vitro by phorbol esters. Suitable for studies of PKCδ and its regulation. PKCδ is a Ca2+-independent and phospholipid-dependent enzyme.


Packaging

Please refer to vial label for lot-specific concentration.


Physical form

In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON™ X-100 Detergent, pH 7.5.


Preparation Note

Following initial thaw, aliquot and freeze (-70°C).


Other Notes

Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329.
Kazanietz, M.G., et al. 1993. Mol.Pharmacol. 44, 298.
Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.
One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PKCε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
assay≥95% (SDS-PAGE)
Quality Segment100
formliquid
specific activity≥800 units/mg protein
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze,avoid repeated freeze/thaw cycles
shipped inwet ice
storage temp.−70°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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