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- 规格参数
- 包装参数
General description
Cilia- and flagella-associated protein 43 (UniProt: E9Q7R9; also known as CFAP43) is encoded by the Cfap43 gene (Gene ID: 100048534) in murine species. CFAP43, a flagellar protein with six WD repeats, is expressed in testis, lung, brain, oviduct, and nasal cavity. It is detected very early during embryonic development It is involved in the regulation of the beating frequency of motile cilia on the epithelial cells. It is also involved in the formation of flagellar axoneme organization and function during spermatogenesis. Mutations in Cfap43 gene have been linked to male infertility. Any malfunction of motile cilia, resulting from defective CFAP43, cause a variety of developmental defects in humans and animal models. These defects may also include impaired mucociliary clearance of airways and hydrocephalus. Cfap43 knockout mice (-/-) exhibit dilation of the third and lateral ventricles. (Ref.: Rachev, E. (2020). Dev. Biol. 459(2); 109-125; Morimoto, Y., et al. (2019). Neurology. 92(20); e2364-e2374; Coutton, C., et al. (2018). Nature Commun. 9; Article 686).
Immunogen
MBP-tagged recombinant fragment corresponding to 174 amino acids from the C-terminal half of mouse Cilia- and flagella-associated protein 43 (CFAP43).
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse testis tissue extracts.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP43 in Mouse testis tissue extracts.
Tested Applications
Western Blotting Analysis: A representative lot detected CFAP43 in Western Blotting applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).
Immunofluorescence Analysis: A representative lot detected CFAP43 in Immunofluorescence applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CFAP43 in Immunoprecipitation applications (Rachev, E., et al. (2020). Dev Biol. 459(2):109-125).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 27D5 is a mouse monoclonal antibody that detects Cilia- and flagella-associated protein 43. It targets an epitope within the C-terminal half.
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 27D5, monoclonal |
| mol wt | calculated mol wt 193.43 kDa,observed mol wt ~190 kDa |
| purified by | using protein G |
| species reactivity | mouse |
| packaging | antibody small pack of 100 μL |
| technique(s) | immunofluorescence: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2b |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_081835 |
| UniProt accession no. | E9Q7R9 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cfap43(951475) |
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