N-Acetylneuraminic Acid Aldolase from microorganisms,25UNITS,A6680-25UN,Sigma

销售价: ¥ 10521.07 / 件
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订货号 : 0BT7321
品牌型号 : Sigma A6680-25UN
货期 : 询货期
最小订货量 : 1件
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产品介绍 Product Description

General description

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)


Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Used in the Sialic Acid Quantification Kit, SIALIC-Q


Physical form

Lyophilized powder containing mannitol and EDTA


Other Notes

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.


技术参数 Specifications
formlyophilized powder
Quality Segment200
specific activity≥20 units/mg protein (biuret)
mol wt ~98 kDa
storage temp.−20°C
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宽度(mm)
高度(mm)
重量(kg)
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