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General description
mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel. Alpaca derived anti-mCherry VHH single domain antibody (molecular weight: 14.3 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Immunogen
Recombinant full length mCherry protein
Application
ELISA, WB, IF
Biochem/physiol Actions
mCherry
Packaging
50 µg per vial / 100 µg per vial
Physical form
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.11
Preparation Note
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Stable for 1 year at 4°C from date of receipt.
Other Notes
Storage at 4°C; Do not freeze.
| biological source | alpaca |
| Quality Segment | 100 |
| recombinant | expressed in E. coli |
| conjugate | unconjugated |
| antibody form | affinity purified VHH antibody |
| clone | RP55, recombinant monoclonal |
| assay | >95% (SDS-PAGE) |
| form | lyophilized solid |
| mol wt | 14.3 kDa, ~15-18 kDa (SDS PAGE under reducing condition) |
| purified by | immobilized metal affinity chromatography (IMAC) |
| concentration | 1 mg/mL |
| technique(s) | ELISA: 1:2000-1:10000,immunofluorescence: 1:500-1:2000,western blot: 1:1000-1:5000 |
| color | colorless |
| pH | ( in PBS, pH 7.4) |
| isotype | VHH |
| Protein ID accession no. | UFQ89828.1 |
| UniProt accession no. | A0A4D6FVK6 |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
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