Glyoxalase I from Saccharomyces cerevisiae,2500UNITS,G4252-2.5KU,Sigma

销售价: ¥ 10758.65 / 件
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订货号 0BQ8125
品牌型号 Sigma G4252-2.5KU
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.


Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.


Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.


Physical form

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5


Analysis Note

Protein determined by biuret.


Other Notes

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.


技术参数 Specifications
typeGrade IV
Quality Segment200
formbuffered aqueous glycerol solution
specific activity≥400 units/mg protein
foreign activityglyoxalase II ≤1%
storage temp.2-8°C
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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