- 商品介绍
- 规格参数
- 包装参数
General description
Nidogen-1 belongs to the nidogens family of glycoproteins. This family consists of nidogen-1 and nidogen-2, both of which are sulfated monomeric glycoproteins. Nidogen-1 is ubiquitously present in the synaptic and extra synaptic basal lamina. It is mapped to human chromosome 1q42.3. Nidogen-1 encodes a 139 kDa, three globular domains namely G1, G2 and G3 and has multiple epidermal growth factor repeats. Recombinant human nidogen-1 is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 133.5 kDa (amino acids 29-1247). This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
Application
This recombinant human nidogen-1 product can be used as an attachment factor for cells adherence.
Biochem/physiol Actions
Nidogen-1 (NID-1) is thought to have an important role in the construction of basement membrane by linking between the networks of laminin and collagen IV. Genetic elimination of the nidogen-1 binding site on the mouse laminin γ1 chain leads to impaired deposition of basement membranes and hampers microvessel maturation. Nidogen 1 and 2 double knockout in mouse fibroblasts led to the loss of all major basal membrane components from the epidermal-collagen interface, and complete abolishment of hemidesmosomes. Supplementation by either recombinant nidogen-1 or -2 improved basal membrane formation. Mutations in NID1and laminin γ1 is implicated in pathogenesis of Dandy-Walker spectrum disorders.
| recombinant | expressed in HEK 293 cells |
| assay | 95% (SDS-PAGE) |
| form | lyophilized powder |
| technique(s) | cell culture | mammalian: suitable |
| storage temp. | −20°C |
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¥ 1125.70
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¥ 503.76
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¥ 580.62
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¥ 3317.36
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¥ 3895.14
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¥ 1153.44
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¥ 298.24
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¥ 634.68
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¥ 1558.44
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¥ 1845.85
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¥ 1101.77
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