- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Bacterial or Yeast Strain: | Mach1™ |
| Cloning Method: | TOPO®-TA |
| Product Line: | One Shot™ |
| Vector: | TOPO-TA Cloning Vectors |
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储存 |
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Box 1: • Topoisomerase I-activated pCR™II-TOPO® vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot® Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C. |
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描述 |
TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR™-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO® Vectors include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR™2.1-TOPO®) or T7 and SP6 (pCR™II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
TOPO® TA Cloning® Kits are also available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.
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| 重量(kg) |
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询价
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询价
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¥ 1056.74
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¥ 989.00
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询价
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¥ 271.60
-
¥ 145.30
-
¥ 3189.90
-
¥ 1627.65
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询价
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¥ 904.40
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¥ 1506.47
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¥ 295.64
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¥ 766.82
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¥ 1680.73
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¥ 145427.01
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¥ 108621.02
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询价
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¥ 22137.02
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¥ 23.20
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