RNeasy 96 Kit (12)，74182，Qiagen，凯杰

RNeasy Kits are the gold standard for total RNA isolation. They provide fast purification of high-quality RNA from small to large amounts of cells, tissues, and yeast using silica membrane RNeasy spin columns or 96-well plates. Tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor II or TissueLyser II or LT system. The RNeasy 96 Kit enables high-throughput purification of total RNA from up to 96 cultured cell samples using silica membrane RNeasy 96 plates. A dedicated RNeasy QIAcube Kit enables automated purification of 1–12 samples on the QIAcube Connect. The QIAwave RNA Mini Kit is an eco-friendlier version of our standard RNeasy Mini Kit. Reducing plastic and cardboard usage by up to 60% and 57%, respectively, this version incorporates waste tubes made from 100% post-consumer recycled plastic that can be reused during the procedure. The QIAwave buffers are provided as concentrates, decreasing plastic use by up to 90% per bottle. Despite the visual differences, the QIAwave Kit remains user-friendly, with chemistry and performance identical to the standard kit's. In partnership with My Green Lab, we've also assessed the environmental impact of the RNeasy Mini Kit and the QIAwave RNA Mini Kit. My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria. These include:  Manufacturing Responsible chemical management Sustainable content within products and packaging materials Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact (see figures "RNeasy Mini Kit ACT environmental impact factor label  US ( 50 / 250 ),  EU ( 50 / 250 ) and  UK ( 50 / 250 )" and “QIAwave RNA Mini Kit ACT environment impact factor label  US ( 50 / 250 ),  EU ( 50 / 250 ) and UK ( 50 / 250 ).  

• Fast procedure delivering high-quality total RNA in minutes
• Ready-to-use RNA for high performance in any downstream application
• Consistent RNA yields from small to large amounts of starting material
• No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
• High-throughput processing in 96-well format and automatable protocols

RNA purified with RNeasy and QIAwave RNA technology has A_260/280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5) and is ideal for use in all applications. Downstream applications include:

• RNA-seq
• Quantitative, real-time RT-PCR
• Real-time RT-PCR starting with as little as one cell
• End-point RT-PCR
• Northern, dot, and slot blotting
• Array analysis
• Poly A+ RNA selection