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- 规格参数
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General description
Apolipoprotein is a polymorphic plasma protein that exists as three isoforms: ApoE4, ApoE3, and ApoE2, which differ in the combination of cysteine and arginine residues located at position 122 and 158. The 35 kDa ApoE4 isoform contains arginine at both locations, and is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Like other isoforms, ApoE4 is a ligand for low density lipoprotein receptors (LDL) and plays a critical role in the transport and metabolism of lipids. In addition to its role in maintaining cardiovascular function, ApoE4 is also critically involved in neuronal plasticity, synaptogenesis, aging, and neurodegenerative disorders. Mice deficient in ApoE4 show age-dependent abnormalities in synaptic structure and impaired long term potentiation. Additionally, ApoE4 has a well-documented role in the pathology of Alzheimer’s disease.
~35 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human ApoE4.
Application
Immunohistochemistry Analysis: A 1:2,000-4,000 dilution from a representative lot detected ApoE4 in human Alzheimer′s diseased brain and human cerebellum tissues.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect ApoE4 in CHO cells secreting ApoE4. (Image courtesy of Dr. Egon Ogris, Medical University of Vienna)
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti--Apolipoprotein E4 antibody is validated for use in IP, WB, IH for the detection of ApoE4.
Biochem/physiol Actions
This antibody recognizes ApoE4 but not ApoE3 or ApoE2.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Recombinant Apolipoprotein E protein
Evaluated by Western Blot in recombinant ApoE4 protein.
Western Blot Analysis: A 1:100,000 dilution detected ApoE4 in 10 µg of recombinant ApoE4 protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 4E4, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000032 |
| UniProt accession no. | P02649 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... APOE(348) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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