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General description
Inhibitor of growth protein 5 (UniProt Q8WYH8; also known as p28ING5) is encoded by the ING5 gene (Gene ID 84289) in human. ING5 is a member of the ING family of proteins known for their antiporliferation activity. ING proteins bind to methylated core histones through their PHD motif (a.a. 186-235 in ING5), and ING5 is known to target histone H3 on di- or trimethylated Lys4 (H3K4me2/3). ING5 associates with two histone acetylase (HAT) complexes, HBO1 and nucleosomal H3-specific MOZ/MORF, that interact with the MCM helicase and are essential for DNA replication during S phase. ING5 deregulations are reported in various cancers and ING5 located chromosome region is deleted in oral squamous cell carcinoma and odontogenic tumors. In addition, ING5 cytosolic localization is reported in some head and neck squamous cell carcinoma tumors and the cytosolic localization positively correlates with the progression of gastric cancer. ING5 also pormotes p53-dependent gene transcription by enhancing p53 acetylation in a p300-dependent manner. ING5 is shown to interact with p63 that mediates the self-renewal of epidermal stem cells, and ING5 knockdown causes differentiation of epidermal stem cells. Similar to other ING proteins, ING5 possesses a PHD (plant homeodomain) type zinc finger, an NCR (novel conserved region), a LZL (leucine zipper like) finger, and two NLS (nuclear localization sequence) motifs.
~31 kDa observed. 27.75 kDa (human ING5 isoform 1; UniProt Q8WYH8-1) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Linzen, U., et al. (2015). PLoS One. 10(4):e0123736). Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST-tagged recombinant human ING5 (Linzen, U., et al. (2015). PLoS One. 10(4):e0123736).
Application
Detect ING5 using this rat monoclonal Anti-ING5, clone 7A11 Antibody, Cat. No. MABS1293, validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ING5 nuclear localization in HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated both Thr152-phosphorylated and non-Thr152-phosphorylated ING5, as well as exogenously expressed wild-type and T152A ING5 constructs (Linzen, U., et al. (2015). PLoS One. 10(4):e0123736).
Western Blotting Analysis: A representative lot detected ING5 in HCT116 and U2OS cell lysates, as well as downregulated ING5 in lysates from ING5 shRNAs-transfected HEK293 cells (Linzen, U., et al. (2015). PLoS One. 10(4):e0123736).
Research Category
Signaling
Biochem/physiol Actions
Clone 7A11 detected shRNA-mediated ING5 downregulation in HEK293 cells (Linzen, U., et al. (2015). PLoS One. 10(4):e0123736).
Physical form
Format: Purified
Protein G purified.
Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected ING5 in 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7A11, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_115705 |
| UniProt accession no. | Q8WYH8 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... ING5(84289) |
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