Anti-APC Antibody, NT, clone Ali 12.28，100UG，MAB3785，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.

Immunogen

Epitope: N-terminus
N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.
Western blot: 4 μg/mL

Immunohistochemistry (Acetone Fixed, Frozen)

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Analysis Note

Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines

Other Notes

Replaces: AB1268

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	mouse
Quality Level	100
conjugate	unconjugated
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	Ali 12.28, monoclonal
species reactivity	human
should not react with	mouse
manufacturer/tradename	Chemicon^®
technique(s)	ELISA: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable
isotype	IgG1
NCBI accession no.	NM_000038.3
UniProt accession no.	P25054
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	human ... APC(324) mouse ... Apc(11789)

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