Anti-F4/80 (mouse), APC, clone BM8.1 Antibody,25UG,MABF1523,Sigma

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订货号 0BW0743
品牌型号 Sigma MABF1523
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.


Immunogen

Immunogen information for this antibody is proprietary information.


Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-F4/80 Antibody (mouse), APC, clone BM8.1, Cat. No. MABF1523 is validated for use in Flow Cytometry, for the detection of F4/80.


Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography


Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.


Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected F4/80 in one million C57Bl/6 splenocytes stained with FITC Anti-mouse CD11b.


Other Notes

Concentration: Please refer to lot specific datasheet.


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerat
Quality Segment100
conjugateAllophycocyanin conjugate
antibody formpurified antibody
antibody product typeprimary antibodies
cloneBM8.1, monoclonal
species reactivitymouse
packagingantibody small pack of 25 μg
technique(s)flow cytometry: suitable
isotypeIgG2aκ
UniProt accession no.Q61549
target post-translational modificationunmodified
Gene Informationmouse ... Adgre1(13733)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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