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Firefly Luciferase Assay，1KIT，SCT154，Sigma

销售价： ¥ 5767.68 / 件

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订货号： 0BW0536

品牌型号： Sigma SCT154

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最小订货量： 1件

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产品介绍 Product Description

General description

Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.

The Firefly Luciferase Assay is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. The luminescence signal decays over the course of about 10 minutes of reaction time, although signal half-life may vary depending on luciferase expression levels.

Application

Firefly Luciferase Assay
Flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.
Research Category
Cell Signaling
Research Sub Category
Live Cell Dye

Physical form

liquid

Preparation Note

Store Firefly Luciferase Assay at -80°C. Firefly Luciferase Assay Buffer 2.0 is stable at -20°C for three months and at –80°C for at least six months from date of receipt. The other kit components are stable at -20°C for at least six months from date of receipt. Kit components and D-luciferin stock solutions in water are stable to at least 5 freeze-thaw cycles.

Other Notes

1) 5X Firefly Luciferase Lysis Buffer (CS224525): 2 X 15 mL

2) Firefly Luciferase Assay Buffer 2.0 (CS224585): 100 mL

3) D-Luciferin (CS224519): 2 X 10 mg

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

technique(s)	cell based assay: suitable
detection method	chemiluminescent
shipped in	ambient
storage temp.	-65 to -96°C

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