Anti-phospho-Gab1 (Tyr627) Antibody,100UL,07-833,Sigma

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订货号 : 0BW0412
品牌型号 : Sigma 07-833
货期 : 询货期
最小订货量 : 1件
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产品介绍 Product Description

General description

115 kDa


Immunogen

Peptide corresponding to amino acid region encompassing the human and mouse phospho-Gab1 (Tyr627)


Application

Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Gab1 (Tyr627) in RIPA lysate of PDGF treated NIH3T3 cells.
Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-phospho-Gab1 (Tyr627) Antibody is validated for use in WB for the detection of phospho-Gab1 (Tyr627).


Biochem/physiol Actions

Gab1 phosphorylated on Tyrosine residue 627.
Predicted to cross-react with human based on 100% sequence homology, but have not been tested.


Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Affinity purified


Preparation Note

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.


Analysis Note

Routinely evaluated by immunoblot.


Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerabbit
Quality Segment100
antibody formaffinity purified immunoglobulin
antibody product typeprimary antibodies
clonepolyclonal
purified byaffinity chromatography
species reactivitymouse
manufacturer/tradenameUpstate®
technique(s)western blot: suitable
isotypeIgG
NCBI accession no.NM_002039.2,NM_207123.1
UniProt accession no.Q13480
shipped indry ice
target post-translational modificationphosphorylation (pTyr627)
Gene Informationmouse ... Gab1(14388)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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