- 商品介绍
- 规格参数
- 包装参数
General description
Recombinant, human WEE1 fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. WEE1 is a tyrosine kinase that causes inhibitory phosporylation of Cdk1 and Cdk2 and acts as a mitotic inhibitor. Protects the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 prior to the onset of mitosis.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Katayama, K., et al. 2005. Mol. Cell. Biol.25, 5725.
Yoshida, T., et al. 2004. Ann. Oncol.15, 252.
Watanabe, N., et al. 1995. EMBO J.14, 1878.
Igarashi, M., et al. 1991. Nature353, 80.
One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)6:2:5:1 per min at 30°C, using variable concentrations of ATP (100 nM to 1.55 µM).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| form | liquid |
| Quality Segment | 100 |
| specific activity | ≥1 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| shipped in | wet ice |
| storage temp. | −70°C |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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¥ 2528.88
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¥ 1563.36
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¥ 2013.69
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¥ 2019.57
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¥ 4039.86
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询价
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询价
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¥ 3442.25
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¥ 1956.41
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询价
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¥ 3812.28
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询价
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询价
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¥ 4897.21
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¥ 5061.38
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¥ 787.34
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¥ 2277.64
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¥ 2452.91
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¥ 2173.94
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