Anti-Beta Galactosidase Antibody, E. coli，100UL，AB3403-I，Sigma 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

General description

β Galactosidase, also known as Beta-galactosidase, Beta-gal, LacZ, or Lactase, and encoded by the gene lacZ, is the enzyme in bacteria that hydrolyzes galactosides (sugars chains containing galactose) in to monosaccharides. β Galactosidase is typically an extracellularly produced enzyme that hydrolyzes the beta-glycosidic bond between galactose and another organic moiety. In E. coli, the β Galactosidase gene is present as part of the inducible lac operon system which is activated in the presence of lactose when glucose level is low. Today, bacterial beta galactosidase is used in molecular biology as a reporter marker to monitor gene expression, and because the enzyme has two functional domains, it forms the basis for α-complementation which is the methodology behind for the blue/white screening of recombinant clones. The presence or absence of an active β-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by β-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid.
~120 kDa observed

Immunogen

Recombinant protein corresponding to E. Coli Beta Galactosidase.

Application

Anti-Beta Galactosidase Antibody, E. coli is a highly specific chicken polyclonal antibody & that targets β-Galactosidase & has been tested in western blotting.

Analysis Note

Evaluated by Western Blotting in TOP10/PCU19 E. Coli lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Beta Galactosidase in 10 µg TOP10/PCU19 E. Coli lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB3403

技术参数 Specifications

biological source	chicken
Quality Level	100
conjugate	unconjugated
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
purified by	affinity chromatography
species reactivity	bacteria
technique(s)	western blot: suitable
NCBI accession no.	NP_752394
UniProt accession no.	P16278
shipped in	wet ice
target post-translational modification	unmodified
Gene Information	Escherichia coli K12 ... Lacz(945006)

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