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General description
RBP-jk, Recombinant signal binding protein for immunoglobin kappa J region, is a human homolog for the Drosophila gene, suppressor of hairless. RBP-jk is located in the nucleus and is a 60 kDa monomeric protein that is used as a promoter region in notch signaling. Notch signaling is important in cell to cell communication which allows "a differentiating cell to suppress the same differentiation fate in a neighboring cell (the receiving cell). This ensures that the correct number of cells are distributed to distinct cell fates." When RBP-jk is not associated with Notch proteins, it acts as a transcriptional repressor. A disease associated with RBP-jk is the Epstein Barr virus.
~55 kDa observed
Immunogen
Recombinant protein corresponding to human RBP-Jk.
Application
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Lymphoblastoid cell line.
Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using DG75 cells.
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Hippocampal NSC′s (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).
Electrophoretic Mobility Shift Assay: A representative lot detected RBP-Jk using Hippocampal lysate (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-RBP-Jk, clone 1F1 Antibody is validated for use in Immunoprecipitation and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of RBP-Jk.
Physical form
Format: Purified
Protein G Purified
Purified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunoprecipitation in HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RBP-Jk in 0.5 mg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 1F1, monoclonal |
| species reactivity | Drosophila, human |
| technique(s) | ChIP: suitable,electrophoretic mobility shift assay: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_005340 |
| UniProt accession no. | Q06330 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RBPJ(3516) |
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