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General description
348.4 kDa (isoform 1) and 350.8 kDa (isoform 3) kDa calculated
Protein eyes shut homolog (UniProt Q5T1H1; also known as EGF-like protein 10, EGF-like protein 11, Epidermal growth factor-like protein 10, Epidermal growth factor-like protein 11, Protein spacemaker homolog) is encoded by the EYS (also known as C6orf178, C6orf179, C6orf180, EGFL10, EGFL11, SPAM) gene (ORF UNQ9424/PRO34591, Gene ID 346007) in human. Drosophila eyes shut protein is expressed in photoreceptor cells, the central nervous system, and peripheral sensory ganglia, and is essential for the formation of a matrix-filled intra-rhabdomeral space within the fly′s retina ommatidia, the loss of spacemaker (spam) converts an open rhabdom system to a closed one. Human EYS/SPAM gene likewise is found to be expressed in the eye and the spinal cord, and mutations in human EYS result in retinitis pigmentosa. Human EYS protein contains 27 EGF-like repeats and five laminin G-like (LG1-5) domains (between EGF repeats 21 & 21, 21 & 22, 23 & 24, 25 & 26, and after repeat EGF repeat 27). The presence of the EGF-like repeats is indicative of a role in the Notch signaling pathway, while EYS LG1-5 bear resemblance to the three agrin laminin G domains that are known to interact with several proteins, including α-dystroglycan at the muscle surface.
Immunogen
Epitope: EGF-like domains 9-11.
GST-tagged recombinant human EYS fragment corresponding to the EGF-like domains 9-11.
Application
Anti-EYS Antibody, clone 3G10.1 is an antibody against EYS for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected EYS in human and rat retina tissue sections.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS
Biochem/physiol Actions
Expected to react with spliced isoforms 1 and 3, but not isoforms 2 and 4, reported by UniProt (Q5T1H1).
Physical form
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting of EYS recombinant protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected EYS recombinant protein.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3G10.1, monoclonal |
| species reactivity | human, rat |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_001136272 |
| UniProt accession no. | Q5T1H1 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... EYS(346007) |
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