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General description
Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.
~21 kDa observed
Immunogen
Recombinant protein containing the full length human Rac.
Application
Anti-Rac1 Antibody, clone 23A8, Alexa Fluor™ 647 Conjugate is an antibody against Rac1 for use in Immunocytochemistry.
Research Category
Signaling
Research Sub Category
Developmental Signaling
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
Biochem/physiol Actions
Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
Physical form
Affinity purified
Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in HeLa cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 647 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | 23A8, monoclonal |
| species reactivity | mouse, rat, human |
| technique(s) | immunocytochemistry: suitable |
| isotype | IgG2b |
| NCBI accession no. | NM_006908 |
| UniProt accession no. | P63000 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RAC1(5879) |
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