Anti-Ly-6G (mouse), violetFluor® 450, clone RB6-8C5 Antibody,25UG,MABF1483,Sigma

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订货号 0BV8686
品牌型号 Sigma MABF1483
货期 询货期
最小订货量 1件
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产品介绍 Product Description

General description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.


Immunogen

Immunogen information for this antibody is proprietary information.


Application

Anti-Ly-6G (mouse), violetFluor® 450, clone RB6-8C5 Antibody is an antibody against Ly-6G for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology


Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography


Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.


Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.


Other Notes

Concentration: Please refer to lot specific datasheet.


Legal Information

violetFluor is a registered trademark of Tonbo Biotechnologies


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
biological sourcerat
Quality Segment100
conjugatevioletFluor® 450
antibody formpurified antibody
antibody product typeprimary antibodies
cloneRB6-8C5, monoclonal
species reactivitymouse
packagingantibody small pack of 25 μg
technique(s)flow cytometry: suitable
isotypeIgG2bκ
UniProt accession no.P35461
target post-translational modificationunmodified
Gene Informationmouse ... Ly6G(546644)
长度(mm)
宽度(mm)
高度(mm)
重量(kg)
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