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General description
von Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form, which is then cleaved into von Willebrand antigen 2 (aa 23-763) and vWF (aa 764-2813). vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.
~270 kDa observed, 309.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Protease fragment of VWF binding to heparin sepharose from human plasma.
Application
Anti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.
Research Category
Cell Structure
Western Blotting Analysis: 1 µg/mL from a representative lot detected von Willebrand factor (vWF) in lysates from Human umbilical vein endothelial cells (HUVEC).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected von Willebrand factor (vWF) in Human umbilical vein endothelial cells (HUVEC).
Biochem/physiol Actions
Clone IIIE2.34 is a mouse monoclonal antibody that detects human von Willebrand factor. It targets an epitope with in the VWFD3 domain.
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in human platelets cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected von Willebrand factor (vWF) in human platelets cell lysates.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | IIIE2.34, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000543 |
| UniProt accession no. | P04275 |
| target post-translational modification | unmodified |
| Gene Information | human ... VWF(7450) |
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