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General description
Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
~ 60 kDa observed
Immunogen
GST-fusion protein corresponding to residues 1-149 of human Akt1
Application
Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Biochem/physiol Actions
Recognizes Akt1/PKBα.
Physical form
20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.
Format: Purified
Protein G Chromatography
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
Evaluated by immunoprecipitation on 250 μg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | SKB1, monoclonal |
| species reactivity | mouse, rat, human |
| technique(s) | immunoprecipitation (IP): suitable |
| isotype | IgG |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Akt1(11651) |
| 长度(mm) | |
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| 重量(kg) |
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