Anti-α-Actin Mouse mAb (1A4)，500UL，113200-500ULCN，Sigma

General description

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).

• Antibody Target Gene Symbol: ACTA2
• Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
• Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
• Hu Entrez ID: 59 (Related Antibodies: 113200 )
• Rat Entrez ID: 81633

Immunogen

Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

Application

Frozen Sections (1:50-1:75)

Immunoblotting (see comments)

Paraffin Sections (1:50-1:75)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

Analysis Note

Negative Control
Fibroblasts, striated muscle, or myocardium
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

Other Notes

Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)