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- 规格参数
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General description
Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), andorthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
Immunogen
Epitope: transactivation domain
Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to amino acids 236-242 of human LEF-1 and mouse myeloma cells.
Application
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells
Hematopoietic Stem Cells
This Anti-LEF-1 Antibody, transactivation domain, clone 3A12 is validated for use in WB for the detection of LEF-1.
Western blot
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein.
Physical form
Format: Purified
Purified immunoglobulin. Provided as 0.2 μm sterile filtered solution in phosphate buffered saline with 0.08% sodiumazide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1130
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 3A12, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| input | sample type hematopoietic stem cell(s) |
| isotype | IgG1 |
| NCBI accession no. | NM_016269.2 |
| UniProt accession no. | Q9UJU2 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LEF1(51176) |
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