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General description
Stage-specific embryonic antigen-1 (SSEA-1) is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
The unconjugated parent antibody (Catalog No. MAB4301) has an observed MW at 220-240 kDa
Immunogen
F9 teratocarcinoma stem cells (X-irradiated).
Application
Detect SSEA1 using this Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected no SSEA-1 in H9 human embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Glycobiology
Biochem/physiol Actions
This antibody recognizes mouse Stage-Specific Embryonic Antigen-1 (SSEA-1).
Physical form
Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Preparation Note
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Mouse embryonic stem cells (SCR012).
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected SSEA-1 in mouse embryonic stem cells (SCR012).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | CY3 conjugate |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MC-480, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable |
| isotype | IgM |
| NCBI accession no. | NP_002024 |
| UniProt accession no. | P22083 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FUT4(2526) |
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