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General description
CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
Approx. 147 kDa
Immunogen
Mouse lymph node cells.
Application
Anti-CD45 Antibody, clone IBL-5/25 is an antibody against CD45 for use in FC, IH.
Flow Cytometry Analysis: A previous lot detected CD45, clone IBL-5/25 in mouse peripheral blood lymphocytes
Biochem/physiol Actions
Reactivity with other species has not been determined.
This antibody reacts with all isoforms of mouse CD45 which is expressed on all haematopoietic cells except erythrocytes.
FUSION PARTNER: SP2/0 myeloma cell line
Physical form
Format: Purified
Purified rat monoclonal IgG in phosphate buffered saline containing 10 mM sodium azide.
Preparation Note
Store at 2-8°C for 12 months from date of receipt.
Analysis Note
Control
Mouse colon tissue
Evaluated by Immunohistochemistry in mouse colon tissue.
Immunohistochemistry Analysis: 1:100 dilution of this antibody detected CD45 in mouse colon tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL1326
| biological source | rat |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | IBL-5/25, monoclonal |
| species reactivity | mouse |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable |
| isotype | IgG |
| NCBI accession no. | NP_002829.2 |
| UniProt accession no. | Q14761 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Ptprc(19264) |
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