- 商品介绍
- 规格参数
- 包装参数
General description
Rap guanine nucleotide exchange factor 1 (UniProt: Q13905; also known as CRK SH3-binding GNRP, Guanine nucleotide-releasing factor 2, Protein C3G) is encoded by the RAPGEF1 (also known as GRF2) gene (Gene ID: 2889) in human. RAPGEF1 is a ubiquitously expressed protein that regulates multiple cellular activities and is shown to be essential for early embryonic development in mammals. It is involved in cell proliferation, transformation, cytoskeletal organization, and apoptosis. It is induced during skeletal muscle cell and neuronal cell differentiation. Higher expression of RAPGEF1 is observed in adult skeletal muscle and placenta and in fetal brain and heart. Its phosphorylation at tyrosine 504 enhances its activity as Rap guanine nucleotide exchange factor. It has functions that depend on its catalytic activity as well as protein interaction domain. It contains four SH3 binding motifs (aa 281-292; 451-462; 538-549; and 606-617). It binds to SH3 domain of CRK and GRB2 and transduces signals from CRK to activate RAS. It plays a role in the establishment of basal endothelial barrier function and has a role in nerve growth factor-induced sustained activation of Rap1 and neurite outgrowth. Four isoforms of RAPGEF1 have been described that are produced by alternative splicing. (Ref.: Begum, Z., et al. (2018). Mol. Biol. Rep. 45(6); 1809-1819; Sasi Kumar, K., et al. (2015). Biochim. Biophys. Acta. 1853(10); 2629-2639).
Immunogen
GST-tagged recombinant protein fragment corresponding to the Crk binding region of human RAPGEF1 (aa 268-648).
Application
Quality Control Testing
Evaluated by Western Blotting in HEK293 cells transfected with GFP-Crk-binding region (CBR).
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected RAPGEF1 in HEK293 cells transfected with GFP- Crk-binding region (CBR), but not in cells without GFP-CBR transfection.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected RapGEF1 in Immunocytochemistry application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RapGEF1 in Immunoprecipitation application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819).
Western Blotting Analysis: A representative lot detected RapGEF1 in Western Blotting application (Begum, Z., et al. (2018). Mol Biol Rep. 45(6):1809-1819; Sriram, D., et al. (2020). Sci Rep. 10(1):18838).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 3F6 is a mouse monoclonal antibody that detects Rap guanine nucleotide exchange factor 1 (RAPGEF1). It targets an epitope from the internal region.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3F6, monoclonal |
| mol wt | calculated mol wt 120.55 kDa,observed mol wt ~75 kDa |
| purified by | using protein G |
| species reactivity | monkey, rat, human, mouse |
| packaging | antibody small pack of 100 μL |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| epitope sequence | Internal |
| Protein ID accession no. | NP_001291204 |
| UniProt accession no. | Q13905 |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... RAPGEF1(2889) |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 12156.68
-
¥ 1451.54
-
¥ 9207.92
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 8520.40
-
询价
-
询价
-
询价
-
无菌，PETG 方形有刻度培养基瓶，带盖，聚对苯二酸乙二醇酯共聚物，天然高密度聚乙烯盖，500毫升容量，每箱40，40/箱，342020-0500，Nalgene，Thermofisher，赛默飞世尔¥ 3684.34
-
¥ 3852.13
-
¥ 1196.14
-
¥ 5014.59
-
¥ 3896.11
-
¥ 2099.27
-
¥ 10685.48
微信小程序
7X24小时在线咨询