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General description
Transportin 3 (UniProt Q9Y5L0; also known as Imp12, Importin 12, Limb girdle muscular dystrophy 1F, Transportin-SR, TRN-SR, TRN-SR2) is encoded by the TNPO3 gene (also known as IPO12, LGMD1F, MTR10A, TRNSR) (Gene ID 23534) in human. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and is regulated by the small GTPase Ran. Importins bind their cargo in the cytoplasm, and the competitive binding of RanGTP induces cargoes release in the nucleus. Most nuclear importins and exportins, including transportin-3 (importin-12), belong to the beta-karyopherin family of proteins. Transportin-3 associates with and mediates the nuclear import of a wide range of serine/arginine-rich/RS domain-containing proteins, the majority of which are involved in mRNA processing, including the cleavage and polyadenylation specificity factor 6 (CPSF6, CF-Im-68) and the splicing factors alternative splicing factor/splicing factor 2 (ASF/SF2) & SC35. In addition, Transportin-3 also plays a cofactor role in facilitating HIV-1 replication. Phosphorylation of the ASF/SF2 RS domain is required for its interaction with Transprotin-3 HEAT repeat 15. Mutations in the HEAT repeat 15 region of Transprotin-3 likewise are reported to impair its interaction with CPSF6 and its ability to support HIV-1 replication.
~100 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to human Transportin-3.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Transportin-3 Antibody is validated for use in Western Blotting for the detection of Transportin-3 .
Biochem/physiol Actions
Expected to react with all four spliced isoforms.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Staurosporin treated Jurkat cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Transportin-3 in 10 µg of Staurosporin treated Jurkat cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), monkey (based on 100% sequence homology), equine (based on 100% sequence homology), mouse (based on 100% sequence homology), sheep (based on 100% sequence homology), rat (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_036602 |
| UniProt accession no. | Q9Y5L0 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TNPO3(23534) |
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