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General description
The inducible isoform of the enzyme nitric oxide synthase (iNOS, NOS-II) is synthesized by macrophages and many other cell types in response to proinflammatory cytokines and certain other stimuli, such as hypoxia and stress.
Immunogen
C-terminal 19 amino acid peptide from human chondrocyte nitric oxide synthase.
Application
Detect Nitric Oxide Synthase II using this Anti-Nitric Oxide Synthase II Antibody validated for use in WB, IH.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
Western blot: 1:1,000-1:2,500 (ECL).
Immunohistochemistry: 1:2,500-1:6,000 (ABC).
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with nNOS and eNOS by immunohistochemistry. AB5384 reacts with human glia and certain neurons in Alzheimer′s disease by immunohistochemistry.
Physical form
Rabbit serum. Liquid containing 0.05% sodium azide.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NM_000625.3,NM_153292.1 |
| UniProt accession no. | P35228 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NOS2(4843) |
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