首页> 生物试剂> 其他生物试剂> 其他> Anti-AATF Antibody/Rb-binding protein Che-1，100UL，ABC953，Sigma

收藏产品

Anti-AATF Antibody/Rb-binding protein Che-1，100UL，ABC953，Sigma

销售价： ¥ 3306.88 / 件

在线询价

优惠：请询价

订货号： 0BV4842

品牌型号： Sigma ABC953

货期：询货期

最小订货量： 1件

+ -

加入购物车

联系我们

400-920-3909

微信扫一扫，手机采购

精选商品

SLC4A3 100 UL，PA551351，赛默飞世尔

销售价：询价

加入购物车

SC-TOPO TA/ DUAL/ TOP10 25 RXN，K460001SC，Invitrogen

销售价：询价

加入购物车

ANTI-PADI2/PAD2 EA，PA519474，赛默飞世尔

销售价：询价

加入购物车

AVIDIN, NEUTRAVIDIN, TEXAS RED 1 MG，A2665，Invitrogen

销售价：询价

加入购物车

ANTI-MO CD357 DTA-1 PE 100UG，12-5874-82，Affymetrix

销售价：询价

加入购物车

ANTI-RPL11 EA，PA534604，赛默飞世尔

销售价：询价

加入购物车

商品介绍
规格参数
包装参数

产品介绍 Product Description

General description

AATF/Rb-binding protein Che-1, also known as Protein Apoptosis-antagonizing transcription factor (Protein AATF), Rb-binding protein Che-1, and encoded by the gene name AATF/ CHE1/ DED/ HSPC277, is a member of the AATF family. Recent studies have indicated that AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binds to the pocket region of RB1, and consequently it may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. In addition, AATF antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. AATF/Rb-binding protein Che-1 is expressed at high levels in brain, heart, kidney, placenta and thymus.
~70 kDa observed. The calculated molecular weight is 63 kDa, however AATF/Rb-binding protein Che-1 has been shown as a ~70 kDa band in western blots (Guo, G., et al. (2004). J. Biol. Chem. 279:4596-4603).

Immunogen

Epitope: POLR2J domain
Linear peptide corresponding to the POLR2J domain of human AATF/Rb-binding protein Che-1.

Application

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-AATF antibody is validated for use in WB, IC, IP, ChIP for the detection of AATF.
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in U2OS, T98G, LoVo, HT29, MCF7, HeLa, LIPARI, Jurkat, and U2OS cells were immunoprecipitated with anti-Rb (Fanciulli, M., et al. (2000). FASEB. 14:904-912).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in NIH 3T3 transfected cells, Synchronized NIH 3T3, HFF human fibroblasts, and HEK293 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, AT fibroblasts, NIH 3T3 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Immunocytochemistry Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in Synchronized NIH 3T3, HFF human fibroblasts, HEK293, Rb transfected HEK293 cells, and Che-1 transfected NIH3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AATF/Rb-binding protein Che-1 in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

技术参数 Specifications

biological source	rabbit
Quality Segment	100
conjugate	unconjugated
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
technique(s)	ChIP: suitable,immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable
NCBI accession no.	NP_036270
UniProt accession no.	Q9NY61
shipped in	dry ice
target post-translational modification	unmodified
Gene Information	human ... AATF(26574)

长度(mm)
宽度(mm)
高度(mm)
重量(kg)