Ethidium Bromide Adsorber,1EA,331569-1EACN,Sigma

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订货号 : 0BV4620
品牌型号 : Sigma 331569-1EACN
货期 : 询货期
最小订货量 : 1件
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产品介绍 Product Description

General description

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.


Biochem/physiol Actions

Cell permeable: no
Primary Target
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Product does not compete with ATP.
Reversible: no


Preparation Note

When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.


Other Notes

MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103.
Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.


Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Irritant (B)


技术参数 Specifications
Quality Segment200
formsuspension
manufacturer/tradenameCalbiochem®
storage conditionOK to freeze
color gray-brown
shipped inambient
storage temp.15-25°C
SMILES stringC(C)c3c(cccc3)C=C.c2(c(cccc2)C=C)C=C.c1(ccccc1)C=C
InChI1S/C10H12.C10H10.C8H8/c2*1-3-9-7-5-6-8-10(9)4-2;1-2-8-6-4-3-5-7-8/h3,5-8H,1,4H2,2H3;3-8H,1-2H2;2-7H,1H2
InChI keyNWUYHJFMYQTDRP-UHFFFAOYSA-N
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