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General description
Asymmetric dimethylation of arginine side chains in proteins is a frequent posttranslational modification, catalyzed by type I protein arginine methyltransferases (PRMTs). PRMT8 is a membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). PRMT8 can form dimers with PRMT2 or PRMT1 and interacts with FYN kinase. PRMT8 is brain specific and it is a vertebrate restricted paralogue of PRMT1. In the past, arginine methylation was mainly observed on abundant proteins such as RNA-binding proteins and histones, but recent advances have revealed a plethora of arginine-methylated proteins implicated in a variety of cellular processes including signal transduction, epigenetic regulation and DNA repair pathways. PRMT8 has been shown to be critical for proper neuronal development and its expression is regionally restricted in the adult suggesting that PRMT8 is chiefly involved in the somatosensory and limbic systems, and a part of the motor nervous system development and maintenance.
~48 kDa observed
Immunogen
Epitope: C-terminal region of SAM-dependent methyltransferase PRMT-type domain
KLH-conjugated linear peptide corresponding to the SAM-dependent methyltransferase PRMT-type domain of Human PRMT8.
Application
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRMT8 in human and mouse brain tissuse, and mouse thalamus.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Histones
This Anti-PRMT8 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of PRMT8.
Biochem/physiol Actions
Other homologies: Homology: predicted to react with rat.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting using human fetal brain tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected PRMT8 in 10 µg of human fetal brain tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), chicken (based on 100% sequence homology), baboon (based on 100% sequence homology), canine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_062828 |
| UniProt accession no. | Q9NR22 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PRMT8(56341) |
| 长度(mm) | |
| 宽度(mm) | |
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| 重量(kg) |
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