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General description
Aspartate aminotransferase, mitochondrial ((mAspAT), also known as Fatty acid-binding protein (FABP-1) or Glutamate oxaloacetate transaminase 2 or Kynurenine aminotransferase 4 or Kynurenine aminotransferase IV or Kynurenine--oxoglutarate transaminase 4 or Kynurenine--oxoglutarate transaminase IV or Plasma membrane-associated fatty acid-binding protein (FABPpm) or Transaminase A, and encoded by the gene name GOT2, plays an essential role in role in amino acid metabolism and facilitates cellular uptake of long-chain free fatty acids. GOT2 is localized to the mitochondrion; however, exposure to alcohol promotes translocation to the cell membrane.
~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human GOT2 expressed in E. Coli
Application
Anti-GOT2, clone 3E9 Antibody is an antibody against GOT2 for use in western blotting & immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GOT2 in PC-3 and SK-BR-3 cells.
Research Category
Signaling
Research Sub Category
Metabolic Hormones & Receptors
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GOT2 in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 3E9, monoclonal |
| species reactivity | mouse, rat, monkey, human |
| technique(s) | immunofluorescence: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P00505 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... GOT2(2806) |
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