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General description
The calculated for Cytokeratin is 62 kDa, however, different isoforms state Cytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).
The cytokeratins are a family of intermediate filaments found in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or more acoring to molecular weight and isoelectric point (Quinlan et al. 1985). Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen′s disease. It does not react with pulmonary adenocarcinoma.
Immunogen
Purified cytokeratin 5 (Wild, 1986) (Mischke, 1987).
Application
Milli-MarkAnti-Cytokeratin 5 Antibody, 6-FITC, clone D5/16B4 is an antibody against Cytokeratin 5 for use in FC.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Biochem/physiol Actions
Antibody recognizes Human Cytokeratin.
Physical form
Protein A purified
Purified mouse monoclonal IgG1 conjugated to FITC in PBS buffer with 15mg/mL BSA and sodium azide at 0.1%.
Preparation Note
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
Analysis Note
Control
A431 cells
Evaluated by flow cytometry using A431 cells
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | FITC conjugate |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | D5/16B4, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000415.2 |
| UniProt accession no. | P13647 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... KRT5(3852) |
| 长度(mm) | |
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