- 商品介绍
- 规格参数
- 包装参数
General description
Disialoganglioside GD2 (GD2) is a glycosphingolipid, which is part of the ganglioside family. Its structure consists of a ceramide (a fatty acid linked to a sphingosine) backbone connected to a complex oligosaccharide chain that includes two sialic acid residues. GD2 is expressed at low levels in normal tissues, primarily on the surface of neurons and peripheral nerve fibers. However, it is highly expressed in certain types of cancer cells, particularly in neuroblastoma, melanoma, osteosarcoma, and small cell lung cancer. GD2 has complex biological functions in tumor cells, including contributing to cellular adhesion and mediating cytotoxic signals. GD2 plays a crucial role in cell-cell interactions, cell signaling, and modulation of the immune response. It is involved in the regulation of cell growth, differentiation, and adhesion. GD2 is also known to participate in signal transduction pathways by interacting with other cell surface molecules. Tumor cells that are positive for GD2 often have enhanced cell survival, are invasive and proliferative. Furthermore, binding of antibodies leads to immune-independent cell death mechanisms. GD2 contributes to T-cell dysfunction, and functions as an immune checkpoint. Therapeutic strategies, including monoclonal antibodies targeting GD2, have been developed to treat cancers. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Machy. P., et.al. (2023). Front Pharmacol. 14:1249929)
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
LAN-1 cells
Application
Quality Control Testing
Evaluated by Immunohistochemistry Analysis in human lung cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ganglioside GD2 in human lung cancer tissue sections.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ganglioside GD2 in IMR-32 cells.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected Ganglioside GD2 in one million IMR-32 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 14G2a is a ZooMAb® recombinant mouse monoclonal antibody that specifically detects human ganglioside GD2.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 14G2a, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 58.88 kDa |
| purified by | using protein G |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable |
| isotype | IgG2a |
| Protein ID accession no. | NP_001469 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
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