Denaturation Solution,1L,N1531-1L,Sigma

销售价: ¥ 660.76 / 件
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订货号 : 0BT5608
品牌型号 : Sigma N1531-1L
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最小订货量 : 1件
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产品介绍 Product Description

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.


Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.


Other Notes

0.5 N NaOH, 1.5 M NaCl
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.


技术参数 Specifications
gradeMolecular Biology
Quality Level200
formsolution
storage temp.room temp
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