- 商品介绍
- 规格参数
- 包装参数
General description
The Lenti CRISPR HPRT1 gRNA Control Transduction Particles (LV02 vector) product is a critical positive control used to monitor transduction efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control uses a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and Ef1a-driven Puromycin resistance casette. This control requires Cas9 be delivered by a separate vector.
Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.
Application
Functional Genomics/Screening/Target Validation
Other Notes
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.
Legal Information
CRISPR Use License Agreement
Lentiviral, WPRE, and Evrogen Licenses
| form | liquid |
| Quality Segment | 200 |
| packaging | vial of 8x25 μL (aliquots) |
| concentration | (5x108 TU/ml (via p24 titering assay)) |
| application(s) | CRISPR |
| shipped in | dry ice |
| storage temp. | −70°C |
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
¥ 4164.19
-
询价
-
¥ 2290.21
-
¥ 9207.92
-
¥ 4039.86
-
询价
-
询价
-
询价
-
¥ 3152.47
-
询价
-
询价
-
¥ 2151.46
-
¥ 2268.97
-
¥ 2307.00
-
¥ 4011.97
-
¥ 3404.94
-
¥ 2173.94
-
询价
-
¥ 64659.66
-
¥ 8072.66
微信小程序
7X24小时在线咨询