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Biochem/physiol Actions
Cell-permeable, BODIPY-tagged C16 fatty acid analog, a green-fluorescent lipid tracer.
BODIPY (dipyrrometheneboron) fluorophores are non-polar, highly photostable, and exhibit a high fluorescence quantum yield, making them suitable for confocal and two-photon fluorescence microscopy, with excitation and emission at 488 nm and 510 - 520 nm. BODIPY-FL-C16 , a palmitate analog, tagged with BODIPY, is efficiently taken up by Caco-2 cells and aligns with L-FABP and markers for cellular organelles, exhibiting strong binding to liver and intestinal fatty acid-binding proteins (with KD values of 270 and 330 nM, respectively) akin to oleic acid, and its fluorescence intensity markedly rises to 0.87 and 0.72 in the presence of L-FABP and I-FABP, respectively. BODIPY-FL-C16 serves as well to track lipid peroxidation in red blood cell membranes.
Disclaimer
Hygroscopic
| Quality Segment | 100 |
| assay | ≥98% (HPLC) |
| form | powder |
| storage condition | desiccated |
| color | , Faint orange to red |
| solubility | DMSO: 2 mg/mL, clear |
| storage temp. | 2-8°C |
| SMILES string | O=C(CCCCCCCCCCCCCCC1=C2C(C)=CC(C)=[N]2B(F)(F)N3C1=C(C)C=C3C)O |
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