- 商品介绍
- 规格参数
- 包装参数
Application
Iodoacetamide-alkyne-C6 may be used as a chemical probe for labeling cysteine residues in proteins, allowing for the attachment of fluorophores or biotin for visualization or enrichment in proteomic studies.
Biochem/physiol Actions
Iodoacetamide-alkyne C6 (IAA-C6, IA-alkyne-C6, IAM-alkyne-C6) is a bifunctional thiol-reactive probe designed for activity-based protein profiling (ABPP) of reactive cysteine residues in live cells. IA-alkyne probe combines an iodoacetamide group (covalent warhead) for cysteine labeling with an alkyne moiety that serves as a click-chemistry handle, enabling subsequent attachment of fluorophores or biotin-linked reporter for visualization or enrichment, respectively. Optimal usage concentrations are ≤3 μM for cellular studies and ~100 μM for lysates and tissue homogenates, facilitating comprehensive proteomic analysis of cysteine-dependent enzyme activities and protein functions.
| Quality Segment | 100 |
| assay | ≥95% (HPLC) |
| form | powder |
| color | white to beige |
| solubility | DMSO: 2 mg/mL, clear |
| storage temp. | -10 to -25°C |
| SMILES string | O=C(CI)NCCCCC#C |
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