BAY-805，5MG，SML3865-5MG，Sigma

Biochem/physiol Actions

Potent and selective dual USP21 deubiquitinase (DUB) inhibitor with anti-cancer efficacy.

BAY-805 is a higly potent and selective USP21 deubiquitinase (DUBs) inhibitor (IC50 (Emax) = 6 nM (95%) by HTRF assay, 2 nM (87%) by Ub-Rh110 assay; Kd = 2.2 nM by SPR-based competition assay; 50/53% inhibition of USP10/USP22 at 10 µM, <31% inhibition against other DUBs) that effectively upregulates cellular NF-κB activation (7-fold increase at 10 µM 20h post BAY-805 treatment by HEK293T-based reporter assay) with good target engagement (EC50 = 17 nM by USP21 HiBiT CESTA assay).