Rat Osteoblasts: Rob, adult，1EACH，R406-05A，Sigma

General description

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ROb from Cell Applications, Inc. have been used to evaluate a novel hydroxyapatite (HAp)/poly(l-lactic acid) (PLLA) nanocomposite nonwoven fabric, coated with calcined HAp nanocrystals as a material for bone regeneration and demonstrated better spreading on the nanocrystal-coated vs. uncoated surface (Yanagida, 2011). Characterization: Positive for bone mineralization.

Application

Model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments.

Biochem/physiol Actions

Bone

Preparation Note

• 1st passage, >500,000 cells in Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 10 population doublings

Analysis Note

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Other Notes

Rat Osteoblast Basal Medium that contains 10% FBS and 10% DMSO