- 商品介绍
- 规格参数
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General description
WD repeat-containing protein 5 (UniProt: P61964; also known as BMP2-induced 3-kb gene protein) is encoded by the WDR5 (also known as BIG3) gene (Gene ID: 11091) in human. WD repeat domain 5 (WDR5) is a highly conserved protein characterized by its WD40 domain structure, which facilitates protein-protein interactions. This protein is a core component of the MLL/SET1 histone H3 lysine 4 (H3K4) methyltransferase complexes, playing a pivotal role in chromatin remodeling and transcriptional regulation. WDR5 is ubiquitously expressed across various tissues, with notable enrichment in the brain, liver, and kidney, and is predominantly localized in the nucleoplasm and chromatin-associated regions. WDR5 contributes to histone modification by positioning the N-terminus of histone H3 for efficient methylation at lysine 4, thereby influencing gene expression and cellular differentiation. It interacts with multiple proteins, including MYC, PAXBP1, and components of the ATAC complex, linking transcriptional machinery to chromatin remodeling pathways. Dysregulation of WDR5 has been implicated in oncogenesis, particularly in cancers such as leukemia and neuroblastoma, where its role in chromatin accessibility and transcriptional control supports tumor progression. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Trievel, R. and Shilatifard, A., (2009), Nat Struct Mol Biol 16, 678 680)
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human WDR5.
Application
Quality Control Testing
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected WDR5 in MCF7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected WDR5 in human and rat spleen tissue lysate, and a 1:10,000 dilution in C2C12 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound with WDR5 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected WDR5 in A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected WDR5 in human testis tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 3N4 is a ZooMAb® rabbit recombinant monoclonal antibody that detects WDR5. It targets an epitope within 16 amino acids from the N-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Other Notes
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 3N4, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 36.59 kDa,observed mol wt ~38 kDa |
| purified by | using Protein A |
| species reactivity | human, mouse, rat |
| species reactivity (predicted by homology) | porcine, feline, horse, monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal half |
| Protein ID accession no. | NP_060058.1, NP_438172.1 |
| UniProt accession no. | P61964 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... WDR5(11091) |
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