GALACTO-LIGHT PLUS 15000 ASSAY EACH,T1009,Applied Biosystems

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订货号 9PM4795
品牌型号 Applied Biosystems T1009
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Assay: β-Gal (lacZ) Assay
Compatible Cells: Mammalian Cells
Form: Liquid
High-throughput Compatibility: High-throughput Compatible
Label Type: Unlabeled
Product Line: Galacto-Light Plus™, NovaBright™
Readout: End Point
Sample Type: Cell Cultures
Substrate: Galacton-Plus®
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
Technique: Enhanced Chemiluminescence
Validated Application: Enzyme Assay
Detection Method: Chemiluminescent
Format: 96-well plate
For Use With (Equipment): Luminometer (Microplate)
Quantity: 15,000 assays
储存
This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C.
描述

The Galacto-Light Plus™ Beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of β-galactosidase in reporter gene assays.

• The Galacto-Light Plus™ Beta-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Ready-to-Use
The Galacto-Light™ Plus™ system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.

Products described herein are guaranteed for 12 months from the date of purchase.

For Research Use Only. Not for use in diagnostics procedures.

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